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If a particular bacteria is unable to ferment glucose, can I conclude that it is unable to metabolize other carbohydrates eg: lactose, maltose, sucrose?
No because those other carbs are disaccharides whereas glucose is a monosaccharide — different enzymes at play.
Save my name and email in this browser for the next time I comment. Principle of Methyl Red MR Test Some bacteria have the ability to utilize glucose and convert it to a stable acid like lactic acid, acetic acid or formic acid as the end product.
Using organisms taken from an hour pure culture, lightly inoculate the medium. Incubate aerobically at 37 degrees C. Following 24 hours of incubation, aliquot 1ml of the broth to a clean test tube.
Reincubate the remaining broth for an additional 24 hours. Add 2 to 3 drops of methyl red indicator to aliquot.
Types and proportion of fermentation products produced by anaerobic fermentation of glucose are one of the key taxonomic characteristics which help to differentiate various genera of enteric bacteria.
Mixed acid fermentation is one of the two broad patterns, butanediol fermentation being another. In mixed acid fermentation, three acids acetic, lactic and succinic are formed in significant amounts.
The mixed acid pathway gives 4 mol of acidic products mainly lactic and acetic acid , 1 mol of neutral fermentation product ethanol , 1 mol of CO 2 , and 1 mol of H 2 per mol of glucose fermented.
These large amounts of acid results significant decrease in the pH of the medium below 4. This is visualized by using pH indicator, methyl red p-dimethylaminoaeobenzene-O-carboxylic acid , which is yellow above pH 5.
The pH at which methyl red detects acid is considerably lower than the pH for other indicators used in bacteriologic culture media.
Thus, to produce a color change, the test organism must produce large quantities of acid from the carbohydrate substrate being used.
MR Positive : When the culture medium turns red after the addition of methyl red, because of a pH at or below 4. MR Negative : When the culture medium remains yellow, which occurs when less acid is produced pH is higher from the fermentation of glucose.
Positive and negative controls should be run after the preparation of each lot of medium and after making each batch of reagent.
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